Xenograft Tumor Growth Assay

Female BALB/c nude mice (4–6 weeks; n = 6/group) were purchased from Shanghai Laboratory Animal Center (Shanghai, China) and raised in specific pathogen-free conditions. SKOV3 cell lines were incubated for 24 h after gas treatment with or without 5.1% sevoflurane stimulation. Then, the SKOV3 cell lines were prepared as a 2 × 10⁶ cells/mL single-cell suspension. The female BALB/c nude mice were hypodermic injected with the cell suspension (0.2 mL/mouse) and divided into 2 groups: Control group and 5.1% (sevoflurane) group. The tumor volume was measured every 3 days, and the tumor volume (V) was calculated according to the formula: V = 1/2ab²(mm³). After 3 weeks, the mice were killed by air embolization and the tumor weight was calculated. The use and care of animals was carried out under the guidelines approved by the Institutional Animal Care and Use Committee of Beijing Obstetrics and Gynecology Hospital, Capital Medical University.