2.2. Composition of cell culture media

Cells were cultured without (Ctrl) or with one of four increasing Sr concentrations, according to a previous study (Bizelli‐Silveira et al., 2018), defining the group they were allocated to Sr1, the physiological level of Sr in the human serum (11.3 × 10⁻³ mg/L); Sr2 (human seric concentration reached after culture with a common Sr systemic supplementation used by osteoporotic women, 13 mg/L); Sr3, 10 times the Sr2 concentration (130 mg/L); and Sr4, approximately 30 times the Sr2 concentration (360 mg/L). Sr was added as strontium chloride hexahydrate (SrCl₂·6H₂O, Sigma‐Aldrich, St. Louis, USA).