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The Assay of 5-Ethynyl-20-Deoxyuridine (EDU)

QL Qin Liu
HS Hexian Shi
JY Jianbo Yang
NJ Ning Jiang
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A Cell Light EDU DNA imaging kit (Guangzhou RiboBio) was used to the assay of EDU. Briefly, after the cells transfected for 24 h on 96-well plates, the 100 mM of EDU has used to culture the cells for 2 h. The specific steps of cell staining were strictly followed in accordance with the manufacturer’s instructions. A BD Pathway 855 high-content analysis system (BD, San Jose, CA, USA) was used to image and analyze the cells. The percentage of EDU-positive cells was calculated as follows:

EDU cells: The number of EDU-strained cells;

Hoechst cells: The number of Hoechst-strained cells.

Copyright and License information:  ©2019 Liu et al.
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