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Internalization Assays by qRT-PCR
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BCMA+ cells (COS-7-BCMA, U266, or H929) were incubated for indicated times with 200 nM aptamer in serum-free medium. After treatment, cells were washed DPBS or with DPBS 0.5 M NaCl in order remove not internalized aptamers. Binding was evaluated by qRT-PCR and the percentage of internalized aptamers on the total bound was calculated.
Copyright and License information: ©2019 The Authors
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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