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A549 cells were infected in serum-free medium at an MOI of 0.01 and after a 1 h absorption period at 37 °C, overlaid with media containing 0.14% BSA and 1 µg/ml L-(tosyl- amido-2-phenyl) ethyl chloromethyl ketone (TPCK)-treated trypsin (Worthington Biochemicals). Supernatants were harvested at varying times post infection and titrated by plaque assay on MDCK cells as previously described47.
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