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Injection of Blood into the Cranial Vault
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A third burr hole was drilled over the frontal lobe, and a spinal needle was inserted until it touched the bone at the base of the anterior cranial vault. The animal’s blood was aspirated from the arterial line and injected deep into the vault over ~5 s (4–6 mL’s per injection). Physiological recordings were undertaken for up to 5 mins following each injection. Five injections were undertaken. In one animal, saline was used for the last 4 injections, due to blood clotting prior to the injection.
Copyright and License information: ©2019 Dixon et al.
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