2.8. Isolation of Hematopoietic Cells from Zebrafish Embryos and Cytospin Preparation

JT Jiaqi Tan
LZ Lei Zhao
GW Gaoxiang Wang
TL Tongjuan Li
DL Dan Li
QX Qian Xu
XC Xing Chen
ZS Zhen Shang
JW Jue Wang
JZ Jianfeng Zhou
ask Ask a question
Favorite

At 48 hpf, 30 embryos were anesthetized with 0.016% tricaine and placed in PBS. Embryonic tails were removed, and the cells were collected by centrifugation at 800 rpm for 5 min and subjected to Wright's staining.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A