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Southern blot analysis.

KM Kavi Mehta
LL Laimonis Laimins
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Southern blot analysis was performed as previously described (16). Southern blots were stripped using Southern strip buffer (0.1× SSC [1× SSC is 0.15 M NaCl plus 0.015 M sodium citrate] and 0.5× SDS). The probed membrane was placed inside a hybridization system, and 50 ml of Southern strip buffer was added. The membrane was incubated with rotating at 95°C for 20 min twice. Following the second wash, 50 ml of 2× SSC was added to the glass cylinder and the cylinder was allowed to cool to room temperature. Once the cylinder had reached room temperature, hybridization buffer was added and probing and hybridization were performed as described above.

Copyright and License information:  ©2018 Mehta and Laimins.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.

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