Calibration curves were calculated for all the analytes. The limits of detection (LODs) and quantitation (LOQs) were calculated as 3- and 10-fold the standard deviation of the intercept of the calibration curves. The within-day (n = 5) and between-days (n = 5, for five days) precision of the method were evaluated at three different concentrations (0.01, 0.1 and 1 µg mL−1).
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