The mouse microglia BV2 cell line was maintained as described previously. Briefly, BV2 microglial cells were maintained at 37 °C in DMEM supplemented with 10% fetal bovine serum, 50 U/ml penicillin and 50 μg/ml streptomycin in a humidified incubator with 5% CO2 and 95% air. The cells were split or harvested every 3–5 days.
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