Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Adult mouse ventricular cardiomyocytes isolation

SH Sina Hadipour-Lakmehsari
AD Amine Driouchi
SL Shin-Haw Lee
UK Uros Kuzmanov
NC Neal I. Callaghan
SH Scott P. Heximer
CS Craig A. Simmons
CY Christopher M. Yip
AG Anthony O. Gramolini
request Request a Protocol
ask Ask a question
Favorite

All experimental procedures were conducted in accordance with the Animal Care Guidelines approved by University of Toronto Animal Use and Care Committee. Protocol for isolation of adult mouse cardiomyocytes was modified from Ackers-Johnson et al.14. Briefly, Sham-, 2-week TAC-, and 4-week TAC-operated CD1 mice hearts were perfused with EDTA buffer and perfusion buffer before being digested with warmed collagenase II buffer (250 units/mL; LS004176, Worthington Biochemical Corp., Lakewood, NJ, USA). Following dissociation and washing, 3 stages of sedimentation were performed to remove the non-myocyte fraction before being suspended in culture media and plated on laminin-coated glass-bottom dishes. Cells were fixed 2 hours after plating and processed for immunofluorescence experiments.

Copyright and License information: The Author(s) ©2019
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A