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MDA-MB-231 cells were cultured in 2% FBS in the presence or absence of 150 ng/ml rHuPCSK6 for 24 h and stained using an Annexin V-fluorescein isothiocyanate apoptosis detection kit (BD Biosciences), according to the manufacturer's protocol. Cells were detected using a Coulter Epics XL flow cytometer, and DNA content was analyzed with Beckman Coulter EXPO32 ADC software (version 1.2B; both Beckman Coulter, Inc.). The experiments were repeated 3 times in triplicate.
Copyright and License information: ©2018 Wang et al.
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
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