Western blotting with rabbit anti-AVPR1A (Bioss BS-11598R), rabbit anti-ACKR3 (AbCam Ab38089), goat anti-CXCR4 (Abcam Ab1670), rabbit anti-β2-AR (Abcam Ab36956), mouse anti-HA (Bioss bsm-50131M) or mouse anti-FLAG (Sigma-Aldrich F1804) in combination with anti-rabbit, anti-mouse (GE Healthcare) or anti-goat (Sigma-Aldrich) IgG horseradish peroxidase-linked whole antibody was performed as described previously [21].
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.