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2.9. Stereological analysis of microgliosis and astrogliosis
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Cell counts were performed every four sections (30 μm-thick) using Fiji Software (http://fiji.sc/Fiji) in 5 sections of the hippocampus separated [32]. The error coefficient attributable to the sampling was calculated according to Gundersen and Jensen (1987), and values ≤ 0.10 were accepted. (n = 4–5 animals per experimental group).
Copyright and License information: ©2019 The Authors
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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