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Senescence associated β-galactosidase (β-gal) assay
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Cells were fixed with 4% formaldehyde for 10 min and incubated overnight at 37°C with 1 mg/ml X-gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside), 5 mM K3Fe(CN)6, 5 mM K4Fe(CN)6, 150 mM NaCl, and 2 mM MgCl2 in 40 mM citric acid/sodium phosphate pH 6.0.
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