2.1. C. guianensis Oil Preparation

The C. guianensis Aublet C. seeds were harvested from December 2013 to February 2014 from under identified parent trees in the Rio de Janeiro Botanical Gardens, with prior authorization. The seeds were superficially cleaned and frozen at −18°C, and for oil extraction, the frozen seeds were first sliced and air-dried at 60°C.

After preparing the sliced and dried seeds, oil 1 was obtained by pressing using a model CA59G expeller-type laboratory press (Komet) with a maximum processing capacity of 5 kg/h of raw material. Screws with 12 mm between the grooves were used, with an oil outlet grid with 1 mm diameter holes and a 10 mm diameter outlet nozzle. The oil obtained was centrifuged at 10,000 rpm for 10 minutes at room temperature to remove particles and then frozen at −18°C.

To evaluate the effects of the thermal treatment, the frozen seeds were autoclaved at 121°C for 15 min and then submitted to slicing, drying, and pressing conditions as presented above to obtain oil 2.

Oil 3 was obtained using the Soxhlet extraction method (30–60°C for 16 h) with petroleum ether, extracting from the dried seeds with no further heat treatment. The solvent was evaporated off in a rotary evaporator under a stream of nitrogen, and the oil obtained was frozen at −18°C.