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Oil Red O staining and quantification of differentiation
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Differentiated adipocytes on 35-mm dishes were fixed with 10% formalin for 10 min, followed by washing with 60% isopropanol for 5 min. The fixed cells were dried, stained with Oil Red O solution (Sigma-Aldrich) for 1 h, and washed four times with water. For quantification, the retained Oil Red O was dissolved in 5 ml (per dish) isopropanol and its absorbance measured at 510 nm in a plate reader.
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