Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
For the BrdU incorporation assay, the BrdU Cell Proliferation Assay (QIA58, Calbiochem, Darmstadt, Germany) was used according to the manufacturers instructions. 3000 cells/well were plated on poly-L-ornithine/laminin-coated 96well plates and BrdU was added for 24 h.
To monitor proliferation, neural precursor cells were cultured as neurospheres plating 500 000 cells per 10 cm dish/passage. The resulting cell number was determined for each passage and the proliferation factor was calculated.
Copyright and License information: ©2016, The Author(s)
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
