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Tissue sections were analyzed using luxol fast blue staining (Sigma, USA). Samples were placed in luxol fast blue overnight at 60 °C and washed with 95% alcohol followed by distilled water to remove the excess blue stain. The blue color was trapped in myelinated white matter after lithium carbonate exposure step (20 sec). Slides were washed three times with 75% alcohol and the background was stained with cresyl violet for 1 min. With this technique myelinated fibers (blue), neutrophils (pink) and nerve cells (purple) were visualized with different colors (15).
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