2.4. MTT Assay and Measurement of IC50

1 × 10⁴ cells/well were seeded in 96-well plates and disposed of increasing concentrations of cordycepin and AECK. After 48 h treatment, MTT working solution was added to 96-well culture plates and incubated continuously at 37°C for 3 h. Discard the culture supernatant and added DMSO to dissolve the formazan crystals. ELISA reader (Molecular Devices, Sunnyvale, CA) was used to measure the absorbance of each well at 540 nm.

100 μL/well increasing concentrations of AECK and 100 μL/well nutrient solution were disposed of in 96-well plates, 200 μL/well in total. Using the method of simple interpolation to calculate IC₅₀