The mouse P19 pluripotent embryonal carcinoma cell line was purchased from the ATCC (Manassas, VA) and maintained in complete MEMα medium (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (ATCC) at 37°C and 5% CO2. The retinol concentration in serum was 25 nM determined by the supplier.
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