Statistics

All statistical analyses were performed on GraphPad Prism 6 statistical analysis package. Data are presented with data points were possible and mean ± SEM or box plots representing interquartile range with whiskers at minimum/maximum. Multiple comparisons were made using ANOVA with Tukey’s post hoc test for pairwise comparisons of normally distributed homoscedastic data. Data were assumed to fit the ANOVA assumptions if the residuals of a dataset were normally distributed. Data that did not meet the ANOVA criteria were analyzed by Kruskal-Wallis with Dunn’s post hoc test. Repeated significance indicator letters (e.g., a–a) signify P > 0.05, while groups with distinct indicators (a vs. b) signify P < 0.05. Letters denote significant differences between groups, P values <0.05 were considered significant, and exact P values for the comparison with highest significant P value is recorded in the figure legend. Comparisons between two datasets were made using Student’s unpaired two-tailed t test, where asterisks denote significant differences where exact P values are reported in the figure legend.