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Clonogenic Cell Survival Assay
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Cells were plated in 6-well plates at 300 cells/well density and cultured overnight before treatments and incubated for 10 days post-treatment. Following the incubation period, cells were washed with 1 × PBS and stained with 0.1% Coomassie blue (Bio-Rad Laboratories S.r.l., Milan, Italy) in 30% methanol and 10% acetic acid. Plates were scanned and the number of colonies was determined using the ImageJ software.
Copyright and License information: ©2019 Cseh, Fabian, Quintana-Cabrera, Szabo, Eros, Soriano, Gallyas, Scorrano and Sumegi.
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