2.10. Carrageenan-Induced Paw Edema in Rats

Carrageenan-induced rat hind paw edema was induced and evaluated, as described previously [30]. Briefly, the group treated with SHF extract was administered 50, 100 and 200 mg/kg for 3 days before the carrageenan injection (1% carrageenan suspended in saline), whereas celecoxib (60 mg/kg, positive control, p.o.) was administered 1 h before the carrageenan injection. Subsequently, 1 h after treatment, 1% carrageenan was injected into the right hind paw of each rat. The left paw was used as a control (0.1 mL saline injected). Paw edema volume was measured before, and at 1, 3 and 5 h after the carrageenan injection, using a plethysmometer (37140, Ugo Basile, Comerio, Italy).