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Energy expenditure and fuel oxidation were assessed using the Oxymax CLAMS (Columbus Instruments). Mice were acclimated in individual chambers for 1 day before data recording. Measurements of in vivo oxygen consumption and carbon dioxide production were performed every 14 min over 2 days and used to calculate the respiratory exchange ratio (RER). Food intake was monitored during the procedure.
Copyright and License information: ©2019 Lian et al.
Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.
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