Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Terminal deoxynucleotidyl transferase dUTP (deoxyuridine 5-triphosphate-digoxigenin) nick end labeling (TUNEL) staining

YG Youguang Gao
TC Ting Chen
XL Xianghui Lei
YL Yunfeng Li
XD Xingui Dai
YC Yuanyuan Cao
QD Qionglei Ding
XL Xiabao Lei
TL Tao Li
XL Xianzhong Lin
request Request a Protocol
ask Ask a question
Favorite

Cortex tissues on histopathological slides were stained using a TUNEL kit (BioVision, Inc., Milpitas, CA, USA) according to the manufacturer's protocols, counterstained with Hoechst 33258, and then examined under an ECLIPSEFN1 fluorescent microscope (Nikon Corporation, Japan). Apoptotic cells appeared fluorescent green, and the number of apoptotic cells per 10 visual fields was determined at ×200 magnification.

Copyright and License information:  ©2016-11-11 Gao et al.
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A