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Cell Line Culture

QZ Qing-Bing Zha
HW Hong-Xia Wei
CL Chen-Guang Li
YL Yi-Dan Liang
LX Li-Hui Xu
WB Wen-Jing Bai
HP Hao Pan
XH Xian-Hui He
DO Dong-Yun Ouyang
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Mouse macrophage cell line J774A.1 was obtained from the Kunming Cell Bank of Type Culture Collection Chinese Academy of Sciences (Kunming, China). The cells were maintained in complete DMEM medium (containing 10% FBS, 100 µg/ml penicillin, 100 µg/ml streptomycin, and 2 mM l-glutamine) and cultured at 37°C in a humidified incubator with 5% CO2. The cells were subcultured every 2–3 days.

Copyright and License information:  ©2016 Zha, Wei, Li, Liang, Xu, Bai, Pan, He and Ouyang.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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