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Cell apoptosis assay and Cleaved Caspase-3 measurement

JC Jiahui Chen
LL Lihui Liu
GW Guifeng Wei
WW Wei Wu
HL Huaxia Luo
JY Jiao Yuan
JL Jianjun Luo
RC Runsheng Chen
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For cell apoptosis quantification, cell viability was determined by staining with Annexin V, Alexa Fluor 488 conjugate (Life Technologies, A13201) and propidium iodide (Life Technologies, P21493) and detected by flow cytometry (BD FACSVERSE) according to the Annexin V flow cytometry protocol. Western blot of the Cleaved Caspase-3 antibody (ABGENT, AP3725a) was measured for the Caspace-3 abundance. The cells were collected for flow cytometry assays or total protein lysate 48 h after transfection.

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