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Fluorescence microscopy

AJ Abhijeet R. Joshi
LH Laura Holtmann
IB Ilja Bobylev
CS Christian Schneider
CR Christian Ritter
JW Joachim Weis
HL Helmar C. Lehmann
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Nine days after treatment with sera, Schwann cell cultures were fixed with 4 % PFA for 15 min, permeabilized with 0.2 % Triton X-100, and incubated with fluorescein-labeled phalloidin (P5282, Sigma) or primary antibody against S100 antibody (Sigma, 1:500) overnight at 4 °C. Cells were incubated with respective secondary antibody to tag S100. Cell nuclei were stained with Hoechst dye. Images of Schwann cells were acquired using a Keyence BZ9000 fluorescence microscope.

Copyright and License information: The Author(s). ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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