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Western blotting was done as described previously in polyacrylamide gradient gels (8–16%; Invitrogen Corporation) run in tris glycine buffer under reduced conditions (Wang et al. 2007). The primary antibodies were smooth muscle actin (A2547; Sigma‐Aldrich, St. Louis, MO) and GAPDH (ab8245; Abcam, Cambridge, MA) The images were quantified, using NIH ImageJ software.
Copyright and License information: ©2018 The Authors. published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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