Plasmid and RNAi

H1.2 and H1.4 cDNAs were cloned into p3 × FLAG-CMV-10, EGFP-C2, pET28b and pGEX-4T3. p300, hMOF, TIP60, HDAC1, HDAC3 and HDAC8 cDNAs were cloned into p3 × FLAG-CMV-10. FLAG-PCAF, GST-PCAF and GST-PCAF-HAT2 (amino acids 352–658) were gifts from Dr Ye Zhang (Chinese Academy of Medical Sciences). FLAG-HDAC2 was a gift from Dr. Jiadong Wang (Peking University Health Science Center). All mutations were generated using a site-directed mutagenesis kit (Vazyme). The following siRNAs were used to silence target genes by transient transfection with Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions:

HDAC1: 5′-UGGCCAUCCUGGAACUGCUAAAGUA-3′;

HDAC2: 5′-GAAGAUCCAGACAAGAGAAUUUCUA-3′;

HDAC3: 5′-CGGGAUGGCAUUGAUGACCAGAGUU-3′;

HDAC8: 5′-GACGGAAAUUUGAGCGUAUUCUCUA-3′.