Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Measurement of ROS
Ask a
question
Favorite
To determine the intracellular levels of ROS, DCFH-DA was utilized. AGS cells were plated in 6-well plates and grown to 60% confluence. AGS cells were treated with 3 µM BQ or OQ for 3, 6, 12 or 24 h. Cells were harvested, stained with 10 µM DCFH-DA for 30 min at 37°C, washed twice with PBS, and then immediately analyzed by flow cytometry (Beckman Coulter, Inc.). ROS levels were analyzed using CytExpert software 2.0 (Beckman Coulter, Inc.).
Copyright and License information: ©2019 Wang et al.
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
