Functional profiling prediction was performed with Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt; version1.1.3) (Langille et al., 2013), and was visualized by STAMP (version 2.1.3) (Parks et al., 2014). Taxonomy composition plot and rarefaction curves were made using R (version 3.4.3) and QIIME.
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