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BCA protein quantitation assay

CN Chee Meng Ng
HL Hwei-San Loh
KM Kasturi Muthoosamy
NS Nanthini Sridewi
SM Sivakumar Manickam
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All BCA assay studies were conducted using a BCA Protein Assay Kit (Thermo Fisher Scientific), while the absorbance of the reacted samples was measured using a UV–visible spectrophotometer (Perkin Elmer Lambda 35; PerkinElmer Inc., Waltham, MA, USA). The absorbance–concentration calibration curve was generated using bovine serum albumin (BSA) standards (Thermo Fisher Scientific). After adding the reagents from the BCA Protein Assay Kit, the samples were incubated for 30 minutes before measuring the absorbance at 562 nm.

Copyright and License information:  ©2016 Ng et al. This work is published and licensed by Dove Medical Press Limited
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