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Cells were removed from the culture dishes, which were then rinsed with PBS and collected into plastic tubes. The pooled cells underwent sonication and then collected in PBS by centrifugation (@ 200 x g for 5 min). Protein samples were stored at −70 °C until assayed. The BCA protein assay kit was purchased from Pierce Chemical Company (Rockford, Ill) and used to determine cell protein concentrations as described earlier [7].
Copyright and License information: ©2019 The Authors
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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