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To complement RNASEH2A‐KO HeLa clones, cells were infected with retroviral supernatant produced in Amphotropic Phoenix packaging cells (Swift et al, 2001) using pMSCVpuro‐based vectors, in the presence of 4 μg/ml polybrene and selected for stable integration using 2 μg/ml puromycin.
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This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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