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Transmission blocking assay (ookinete conversion assay)

CG Christopher Dean Goodman
IA Ingvild Austarheim
VM Vanessa Mollard
BM Bertin Mikolo
KM Karl Egil Malterud
GM Geoffrey I. McFadden
HW Helle Wangensteen
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To assay inhibition of the early mosquito stages of parasite development, P. berghei gametocytes expressing green fluorescence protein (GFP) under the control of the ookinete specific promoter CTRP were induced to undergo in vitro fertilization and subsequent development to ookinete stage in a standard 96-well plate assay, essentially as described [23]. Successful development to ookinete stage was assayed by GFP fluorescence measured using an Enspire fluorescent plate reader (Perkin Elmer).

Copyright and License information: The Author(s) ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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