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Histological (cresyl violet) staining
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For each brain, every tenth coronal section was mounted and air-dried on gelatin-coated slides. Slides were hydrated in graded ethanol solutions, stained with 0.5% cresyl violet (Acros Organics, Somerville, NJ), rinsed in water, dehydrated through graded ethanol solutions, cleared in xylenes (Fisher Scientific, Fair Lawn, NJ), and mounted with Permount (Fisher Scientific).
Copyright and License information: ©2018, Mary Ann Liebert, Inc.
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