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Cytotoxicity Assay

JM Jiajia Meng
GG Gan Gu
PD Pengqin Dang
XZ Xuping Zhang
WW Weixuan Wang
JD Jungui Dai
YL Yang Liu
DL Daowan Lai
LZ Ligang Zhou
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Cytotoxicities of the isolated compounds were tested against human carcinoma cells using the microculture tetrazolium (MTT) assay as described previously (Sun et al., 2017). The tested cell lines included NCI-H460 and A549 (lung carcinoma), BGC-823 (gastric cancer), Daoy (desmoplastic cerebellar medulloblastoma), HepG2 (liver carcinoma), HCT116 (colon cancer), A375 (skin malignant melanoma), MCF-7 (breast cancer), and Capan2 (pancreatic carcinoma). Taxol was used as the positive control. The major compounds (11~14, 16) were screened against A375, A549, MCF-7, Capan2, and HCT116 cells, while the minor compounds (i.e., 1~10, 15, 17) were tested against NCI-H460, BGC823, Daoy, HepG2, and HCT116 cell lines.

Copyright and License information:  ©2019 Meng, Gu, Dang, Zhang, Wang, Dai, Liu, Lai and Zhou.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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