2.6. Determination of platelet aggregation by light transmission aggregometry

Platelet reactivity was determined in platelet‐rich plasma (PRP) obtained from whole blood samples using light transmission aggregometry. Whole blood samples (see above) were centrifuged at 150 g for 10 minutes at room temperature and the supernatant was collected. The pellet was then centrifuged at 1500 g at room temperature for 10 minutes to obtain the platelet‐poor plasma (PPP). Ex vivo platelet aggregation was assessed using a 4 channel aggregometer (Chrono‐log Corporation Model 700; Chrono‐log Corporation). PRP was continually stirred and maintained at 37°C during the assay. The change in light transmission relative to PPP after stimulation with platelet agonists (ADP [20 µmol/L], AA [500 µmol/L], and collagen [2 µg/mL]) was recorded.