Oligonucleotide primer design

Specific primers were designed based on published fsr (GenBank accession No. {"type":"entrez-nucleotide","attrs":{"text":"JN246675","term_id":"360039985","term_text":"JN246675"}}JN246675), efm (GenBank accession No. {"type":"entrez-nucleotide","attrs":{"text":"AF097414","term_id":"3790617","term_text":"AF097414"}}AF097414), esp (GenBank accession No. {"type":"entrez-nucleotide","attrs":{"text":"AH013271","term_id":"1036031880","term_text":"AH013271"}}AH013271 and {"type":"entrez-nucleotide","attrs":{"text":"JF826520","term_id":"334546824","term_text":"JF826520"}}JF826520), cylA (GenBank accession No. {"type":"entrez-nucleotide","attrs":{"text":"JQ794947","term_id":"387308847","term_text":"JQ794947"}}JQ794947 and {"type":"entrez-nucleotide","attrs":{"text":"KY613925","term_id":"1187527000","term_text":"KY613925"}}KY613925), cad1 (GenBank accession No. {"type":"entrez-nucleotide","attrs":{"text":"AF421355","term_id":"19481589","term_text":"AF421355"}}AF421355, NC017960, {"type":"entrez-nucleotide","attrs":{"text":"CP003504","term_id":"392334715","term_text":"CP003504"}}CP003504, and {"type":"entrez-nucleotide","attrs":{"text":"AOSM01000007","term_id":"474483845","term_text":"AOSM01000007"}}AOSM01000007), ace (GenBank accession No. {"type":"entrez-nucleotide","attrs":{"text":"AF159247","term_id":"5442425","term_text":"AF159247"}}AF159247 and {"type":"entrez-nucleotide","attrs":{"text":"KY613927","term_id":"1187527004","term_text":"KY613927"}}KY613927), gelE (GenBank accession No {"type":"entrez-nucleotide","attrs":{"text":"D85393","term_id":"1339878","term_text":"D85393"}}D85393, {"type":"entrez-nucleotide","attrs":{"text":"KT598464","term_id":"1024992754","term_text":"KT598464"}}KT598464, {"type":"entrez-nucleotide","attrs":{"text":"EU423275","term_id":"167599449","term_text":"EU423275"}}EU423275, and {"type":"entrez-nucleotide","attrs":{"text":"KY613931","term_id":"1187527012","term_text":"KY613931"}}KY613931), and asa1 (GenBank accession No. {"type":"entrez-nucleotide","attrs":{"text":"KT598461","term_id":"1024992709","term_text":"KT598461"}}KT598461, {"type":"entrez-nucleotide","attrs":{"text":"KY613929","term_id":"1187527008","term_text":"KY613929"}}KY613929, and {"type":"entrez-nucleotide","attrs":{"text":"U91527","term_id":"2109265","term_text":"U91527"}}U91527) gene sequences using OligoAnalyzer (Integrated DNA Technologies, USA). Primer specificity was confirmed by BLAST searches against the National Center for Biotechnology Information (NCBI) database (https://www.ncbi.nlm.nih.gov/). The eight pairs of primers and amplicon sizes are listed in Table 1.