2.11. IKKβ kinase activity assay in vitro

IATL‐mediated inhibition of IKKβ kinase activity was assessed in vitro using a cell IKKβ kinase activity spectrophotometry quantitative detection kit. Briefly, IATL‐treated U87 cells were harvested and lysed with the lysate in the kit. After the protein was quantified, 10 μL of the sample solution (containing 50 μg of protein) was mixed with the reaction solution in the kit. The total activity and nonspecific activity were measured using a microplate reader. The data were evaluated according to the formula in the manual, and the specific activity value was calculated (specific activity = total activity‐nonspecific activity).