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Measurements of cytokine production using ELISA
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Supernatants were collected from monocytes which had been stimulated with various concentrations of Gal-4 or LPS for 24 h or 48 h. The concentrations of IL-1β or IL-12 in the culture supernatants were determined with ELISA kits (both from eBioscience Inc., San Diego, CA, USA) according to the manufacturer's instructions. Absorbance at 450 nm was measured by a PerkinElmer Plate Reader (PerkinElmer, Waltham, MA, USA).
Copyright and License information: ©2019 2019. The Korean Association of Immunologists
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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