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Western blot analysis and kinase activity assays
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Whole-cell extracts were prepared in buffers containing phosphatase and protease inhibitors and used for Western blot analysis as previously described16,50,51. The proteins were subjected to electrophoresis alongside comparative molecular weight markers (GE Healthcare) to determine the size of the protein product. Non-radioactive JNK activity assays were carried out using kits from Cell Signaling Technology according to the manufacturer’s instructions.
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