Sample preparation

JL Jiliang Liu
JK Jeong Im Kim
JC Joanne C. Cusumano
CC Clint Chapple
NV Nagarajan Venugopalan
RF Robert F. Fischetti
LM Lee Makowski
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Wild-type and lignin biosynthetic mutants of Arabidopsis were grown for 6 weeks under long day condition (16 h light/8 h dark), and the bottom of primary stems were cut into 100-µm-thick longitudinal sections. One sample set was immersed in water at room temperature for 3 days and subsequently dehydrated in air at room temperature for 24 h before data collection. A second set was immersed in water at room temperature for 30 days prior to being dehydrated.

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