Morris water maze task

The Morris water maze test is a behavioral test used to evaluate the effect of BG on cognitive enhancement. A black circular pool with a diameter of 150 cm and a depth of 60 cm was filled with water at 23±1°C and the pool was split into 4 quadrants. On first day of trial, mice were inserted into one of the water pool guadrants without a platform for 2 min for free swimming as adaptation training. During the next 4 days of training, a platform (10 cm diameter) was placed 1 cm below the water surface in one quadrant of the pool. Mice were inserted into each quadrant of the pool were trained to find the platform; the time to find the platform was recorded. When the mice reached the platform, they could stay for 20 s. If the mice did not find the platform within 120 s, the mice were placed on the platform for 10 s to remember. On the last day of the trial, platform was hidden; the time taken for the mice to first pass the platform and the number of times the mice crossed the platform were recorded. After each trial, mice were dried with a towel. Mice were administrated with scopolamine 30 min before the trail and with tacrin and BG 1 h before the trial.