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3.4. Preparation of Two-Phase Solvent System and Sample Solution

XZ Xuezhen Zhou
MY Mengqi Yi
LD Lijian Ding
SH Shan He
XY Xiaojun Yan
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The HSCCC experiments were performed using a two-phase solvent system comprising n-hexane/ethyl acetate/methanol/water (2:8:3:7, v/v/v/v) solvent. The two phases were separated after thoroughly equilibrating the mixture in a separating funnel at 25 °C. The upper organic phase was used as the stationary phase, and the lower aqueous phase was employed as the mobile phase.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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