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Soil was sampled in mid-August 2017 from the Risöfladan experimental field located in Vaasa, Finland (63° 02′ 50.22″N, 21° 42′ 41.85″E; Figs 1 and and2).2). Ten replicate soil samples of 5 g were taken from 75 cm below ground (oxidized zone, OX), 140 cm below ground (transition zone, TR), and 190 cm below ground (un-oxidized zone, UN). The samples were placed in separate sterile tubes (30 tubes in total), immediately preserved by freezing in liquid nitrogen, and stored at −80 °C until RNA and DNA were extracted within ten days of sampling.
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