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Transformants on YPD selection agar plates were replicated and grown on new agar plates at 30°C. Plasmid DNA was extracted from each replica transformant using a Clontech Easy Yeast Plasmid Isolation kit (TaKaRa Bio). The extracted plasmid DNA was used to transform E. coli HST08 premium competent cells (TaKaRa Bio).
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