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Western blot

YG Yixue Gu
HL Hao Liu
FK Fangren Kong
JY Jiahui Ye
XJ Xiaoting Jia
ZZ Zhijie Zhang
NL Nan Li
JY Jiang Yin
GZ Guopei Zheng
ZH Zhimin He
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The total proteins were extracted from the corresponding cells, loaded and separated on 10% SDS-PAGE and then transferred to PVDF membranes (Millipore, Billerica, MA, USA). The primary antibodies used for the analysis included mouse anti-KAT6B monoclonal Ab (Abnova, Taiwan, China); rabbit anti-phospho-PI3K p85 (Tyr458) polyclonal Ab, mouse anti-phospho-Akt (Thr308) monoclonal Ab, rabbit anti-PTEN monoclonal Ab and mouse anti-κBα (Ser32/36) monoclonal Ab (Cell Signaling Technology, Danvers, MA, USA); mouse anti-tubulin monoclonal Ab (Santa Cruz, Dallas, Texas, USA).

Copyright and License information: The Author(s). ©2018
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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